high 129 sensitivity human adiponectin elisa kit  (BioVendor Instruments)

Journal: The Journal of Veterinary Medical Science

Article Title: Hematological, biochemical and hormonal profiles in dogs with obesity-related metabolic disorder

doi: 10.1292/jvms.25-0381

Figure Lengend Snippet: Serum concentrations of leptin (A), adiponectin (B), resistin (C), insulin (D), Homeostasis model assessment for insulin resistance (HOMA-IR) (E) and Homeostasis model assessment for beta-cell (HOMA-β) (F) in obesity-related metabolic disorder (ORMD) negative (white bars) and positive (grey bars) obese dogs.

Article Snippet: Adiponectin levels were evaluated with a high-sensitivity human adiponectin ELISA kit (RD191023100; BioVendor R&D, Brno, Czech Republic), which has been validated for use in dogs [ ].

Techniques:

Journal: Molecular Therapy

Article Title: Reversion of metabolic dysfunction-associated steatohepatitis by skeletal muscle-directed FGF21 gene therapy

doi: 10.1016/j.ymthe.2024.10.023

Figure Lengend Snippet: Reversal of WAT hypertrophy and inflammation by AAV1-FGF21 treatment (A) Immunohistochemical analysis of MAC2 in eWAT sections. Red arrows indicate crown-like structures. Scale bars, 100 μm. (B) Morphometric analysis of the mean adipocyte area in eWAT ( n = 4/group). (C and D) Weight of eWAT (C) and iWAT (D) depots ( n = 5–10/group). (E and F) Serum leptin (E) and adiponectin (F) levels ( n = 5–10/group). (G–I) Expression levels of the inflammatory markers Cd68 (G), F4/80 (H), and Tnfa (I) in eWAT ( n = 5–10/group). Data are presented as mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by one-way analysis of variance (ANOVA) with Bonferroni multiple comparison test. FC, fold change; w, weeks.

Article Snippet: Canine adiponectin in serum was measured using the Human adiponectin ELISA High sensitivity kit (RD191023100, BioVendor Group), which has been previously validated for reliable measurement of canine adiponectin., Serum FGF21 levels in dogs remained undetectable when measured with four different canine FGF21 ELISAs (E08F0016, Blue Gene; MBS028669, MyBiosource; ABIN1053687, Antibodies-online; KT-100218, Kamiya Biomedical Company).

Techniques: Immunohistochemical staining, Expressing, Comparison

Journal: Molecular Therapy

Article Title: Reversion of metabolic dysfunction-associated steatohepatitis by skeletal muscle-directed FGF21 gene therapy

doi: 10.1016/j.ymthe.2024.10.023

Figure Lengend Snippet: AAV1-FGF21 biodistribution and biological activity in dogs Healthy Beagle dogs (dog-1 and dog-2) were treated with 7 × 10 12 vg/kg of AAV1-canine optimized FGF21 and followed up for 4 months. (A) Vector genome copy number (left panel, blue) and canine optimize FGF21 ( coFGF21 ) expression (right panel, orange) were analyzed in tissue punches from multiple regions of the skeletal muscle and the liver obtained during necropsy of dogs. (B) Schematic representation indicating the mean coFGF21 expression in hindlimb skeletal muscles. Image courtesy of IMAIOS️ (Micheau A, Hoa D, e-Anatomy, www.imaios.com , https://doi.org/10.37019/e-anatomy ). (C) Levels of biologically active FGF21 in fasted conditions measured using a cell-based reporter gene assay (iLite). (D) Hepatic expression levels of KLB ( n = 2–3/group). (E) Serum triglyceride levels pre-AAV and 4 months post-AAV ( n = 1–3). (F and G) Hepatic expression levels of ACADM (F) and ACADL (G) ( n = 2–3/group). (H) Serum adiponectin levels pre-AAV and 4 months post-AAV ( n = 1–3). (I–L) Quantification of ADIPOQ (I), UCP1 (J), ELOVL3 (K), and PPARGC1A (L) expression in perirenal (prWAT) and gluteal WAT (gWAT) ( n = 2–3/group). (M) Representative images of the hematoxylin and eosin staining of prWAT sections. Insets show multilocular adipocytes in prWAT of AAV-FGF21-treated dogs. Scale bars, 100 μm. Inset scale bars, 25 μm. Data are presented as mean ± SD. Data were analyzed using a Mann-Whitney two-tailed test. coFGF21, canine optimized FGF21; ND, non-detected; vg/dg, vector genomes/diploid genome; AU, arbitrary units; FC, fold change; G, gluteus; T, tensor; Q, quadriceps; V, vastus; LLL, left lateral lobe; LML, left medial lobe; RML, right medial lobe.

Article Snippet: Canine adiponectin in serum was measured using the Human adiponectin ELISA High sensitivity kit (RD191023100, BioVendor Group), which has been previously validated for reliable measurement of canine adiponectin., Serum FGF21 levels in dogs remained undetectable when measured with four different canine FGF21 ELISAs (E08F0016, Blue Gene; MBS028669, MyBiosource; ABIN1053687, Antibodies-online; KT-100218, Kamiya Biomedical Company).

Techniques: Activity Assay, Plasmid Preparation, Expressing, Muscles, Reporter Gene Assay, Staining, MANN-WHITNEY, Two Tailed Test